Development of an Immunochromatographic Strip for Rapid Detection of H9 Subtype Avian Influenza Viruses

Development of an immunochromatographic strip for rapid detection of H9 subtype avian influenza viruses.

An immunochromatographic strip was developed for the detection of the H9 subtype of avian influenza viruses (H9AIVs) in poultry, using two monoclonal antibodies (MAb), 4C4 for H9AIV hemagglutinin (HA) and 4D4 for nucleoprotein. The 4C4 MAb was labeled with colloidal gold as the detection reagent, and the 4D4 MAb was blotted on the test line while a goat anti-mouse antibody was used on the contr...

Assessment of a rapid immunochromatographic assay for the detection of avian influenza viruses

Rapid spreading of the low pathogenic avian influenza virus (AIV) caused by the H9N2 subtype and the highly pathogenic AIV caused by H5N1 have caused serious economic losses in the poultry industries of Asia. Therefore, the early detection of AIVs is crucial for the control of the disease. In the present study, the applicability of a rapid immunochromatographic (RIC) assay, which specifically d...

Immunochromatographic strip assay development for avian influenza antibody detection.

To detect antibody on pen-side is a rapid way to know the avian influenza (AI) infectious status in a chicken flock. The purpose of this study was to develop an immunochromatographic strip (ICS) assay to detect the antibody against the AI virus (AIV) for field applications. The ICS was constructed by fixing an AIV strain A/chicken/Taiwan/2838V/2000 (H6N1) onto a nitrocellulose membrane as the a...

comparison between rt- pcr and viral isolation for detection of h9 subtype of avian influenza viruses

during 2004-2006, a total number of 22 tissue samples obtained from poultry flocks suspected to respiratory diseases submitted to avian virology laboratory of faculty of veterinary medicine, were prepared for avian influenza virus (aiv) isolation in embryonated chicken eggs, according to the standard method. rt-pcr was established on tissue samples using specific primers for h9 gene. amplified ...

Development of an immunochromatographic strip for rapid detection of 2 the H 9 subtype of avian influenza viruses ( H 9 AIVs ) 3